![]() ![]() ![]() It also includes a cloud DVR and optional add-ons from Epix and Starz. Philo offers bread-and-butter cable staples like AMC, Comedy Channel, Nickelodeon and Magnolia Network (formerly DIY), and specializes in lifestyle and reality programming. Philo: This $25 live TV streaming service offers a variety of channels, but it lacks sports channels, local stations and big-name news networks - although Cheddar and BBC news are available.Live TV streaming services we also tested Top channels not available on Sling Orange: ABC, CBS, Fox, NBC, Animal Planet, Bravo, CNBC, Discovery Channel, Bravo, Fox News, Fox Sports 1, FX, MSNBC, USA Network. ABC, Fox and NBC are available in select markets. Top channels not available on Sling Blue: CBS, Animal Planet, Disney Channel, ESPN, Nickelodeon. It does offer a cut-down version called Freestream with a number of included channels. While Sling doesn't have a free trial, the company is offering a $20 discount for your first month. In addition to a relatively affordable price, Sling TV has two new feathers in its cap: an upgraded DVR (increased to 50 hours) and a new interface (as seen above) which makes the service a lot more fun to use. Sling is also `cheaper than most other streaming services, let alone cable. As a result, Sling TV may cost up to $20 more than Philo, but it still has better channels, more options and a comparatively better live TV streaming interface. Meanwhile, Orange & Blue combines the two offerings for $55.įurther, the addition of ABC to five markets has increased its price there to $45 - while it remains $40 in others. ![]() While a number of live TV channels are common to both, Orange is essentially the ESPN/Disney package, while Blue is the Fox/Discovery package. You see, Sling tenders two different $40-ish-per-month live TV streaming channel packages, Sling Orange and Sling Blue. Yet, things are a little more complicated than they are for Sling's competitors. If you're looking for the cheapest live TV streaming service, and one which is still able to offer a usable amount of channels, then it's without a doubt Sling TV Blue. Top channels not available : A&E, History, Lifetime, MLB. Note: YouTube TV is offering the first three months for a $8 discount at $63. While both Sling TV and Hulu are cheaper, YouTube TV is still worth subscribing to if you want tightly integrated search and a great channel selection. Given a lack of 4K content otherwise, it's not a great value for that alone, but it does add an unlimited number of simultaneous streams (up from three) and offline DVR downloads. In addition, the video streaming service has a $20 monthly upgrade that lets you watch 4K livestreams, of college football in particular. In 2023, YouTube TV is the exclusive home of NFL Sunday Ticket, with pricing starting at $249 for early birds. YouTube TV is also the only one to offer surround sound on live broadcasts. The interface is no-nonsense, even a little drab, and yet it offers most of the features a cable service can give you. The basic $73 YouTube TV service also has the best cloud DVR of the bunch, including both unlimited storage and a generous nine months to watch recordings (most rivals offer 30 days). YouTube TV has more top channels than any competitor at this price, and it's one of only two with local PBS stations currently - Hulu will be coming online later in 2023. Top channels not available: AMC, BBC America, MLB Network, NBA TV. Given that Hulu Plus Live TV is now slightly cheaper than YouTube TV, and offers you more, it's now the service to choose for live TV streaming.Īs of December 2022, Hulu Plus Live TV includes the ad-supported version of the Disney Bundle, while the existing ad-free bundle increased to $83 for new subscribers. Hulu's exclusive titles such as The Great, The Handmaid's Tale and Only Murders in the Building, plus its massive catalog of broadcast programming, grant it a content advantage no other service can match. Not only does the $70 service include Hulu basic but also Disney Plus and ESPN Plus, plus an unlimited DVR. Yet, it's Hulu's significant catalog of on-demand content which helps set it apart. Its channel selection may not be as robust as YouTube TV or FuboTV, but it's almost there - especially with the addition of PBS and Magnolia. If you want the best mix of live streaming and on-demand, Hulu Plus Live TV is it. ![]()
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![]() ![]() "Fondly do we hope ~ fervently do we pray ~ that this mighty scourge of war may speedily pass away. And a lot of people were absolutely appalled by that." ![]() "There's the 1619 Project that came in, and that is a rewriting of American history, a false view of American history. She responded by calling the 1619 Project's efforts to document the effects of slavery "evil." "Well, I think sometimes when evil comes into the world and when falsehoods come into our nation, people react to that," Bachmann said. In a July 4 appearance on the Christian program Flashpoint, Bachmann was asked how America's youth viewed the country's inception. Michele Bachmann (R-MN) celebrated America's birthday by declaring that slavery was not a "sinful" part of the nation's founding. Projecting, much? Bachman, Trump, and the rest of the Republican Party have spent years trying to rewrite history by claiming that slavery wasn’t so bad - and now she has the gall to accuse Black historians of rewriting history simply for telling the TRUTH about America’s greatest sin.īREAKING: Trumper Michele Bachmann goes on a shocking rant, declares that the enslavement of millions of Africans in America was not “sinful” - and that the Black historians who are teaching the youth about the centuries of slavery in America are “evil.”īut it gets even worse…… /j7usYYYqD6- Occupy Democrats JFormer Rep. Considering how plaintiffs can now be manufactured by RWNJs, she could raise her standing with the meta-oriented thinkers in the GOP by giving those ‘evil’ “Black historians” subscriptions to the Journal of Historical Review.īREAKING: Trumper Michele Bachmann goes on a shocking rant, declares that the enslavement of millions of Africans in America was not “sinful” - and that the Black historians who are teaching the youth about the centuries of slavery in America are “evil.” But it gets even worse… Bachman then accused today’s Black historians of “rewriting American history,” something that she says is “appalling” to her and other white conservatives, who she claims are “very motivated” to whitewash slavery. Attacking the 1619 Project does seem to be a bit passe. Remember Michele Bachmann and her hubby Marcus, or rather how could we forget, but at least she missed Heritage Month. ![]() ![]() One of the bigger surprises of final cuts was the fact that the Packers kept five outside linebackers, which may tell us how serious Za’Darius Smith’s back injury actually is. Outside Linebacker: Za’Darius Smith, Preston Smith, Rashan Gary, Jonathan Garvin, Chauncey Rivers Willington Previlon, a late riser in the preseason, has a chance to join the practice squad. Heflin is the Packers’ only undrafted rookie who made the team in 2021. The Packers officially keep six defensive linemen after much debate about if Jack Heflin or Tyler Lancaster would be cut. Whenever Bakhtiari returns from the PUP list, the rostered status of Nijman and Hanson may change.ĭefensive Line: Kenny Clark, Kingsley Keke, Dean Lowry, TJ Slaton, Jack Heflin, Tyler Lancaster Expect Ben Braden, who at one point was getting first-team reps at left tackle before Jenkins returned to play, to be considered for the practice squad. Jon Runyan is a hybrid guard-right tackle who was moved in and out of the starting lineup several times throughout the summer. The same is true for Jake Hanson at center. Yosuah Nijman is likely the next man up at left tackle behind Jenkins, after bouncing back from a poor performance in Week 1 of the preseason. Dennis Kelly is a swing tackle who has seen the vast majority of his reps this summer at right tackle. The Packers are going into the season with a starting line of Jenkins-Patrick-Myers-Newman-Turner. Offensive Line: Elgton Jenkins, Lucas Patrick, Josh Myers, Royce Newman, Billy Turner, Jon Runyan, Dennis Kelly, Yosuah Nijman, Jake Hanson (PUP: David Bakhtiari) Defensive end convert Bronson Kaufusi played well as a blocking tight end in the Marcedes Lewis role, making him a priority as a practice squad player. The question now is if Jace Sternberger, a true tight end, will bounce Dominique Dafney, essentially a fullback, off the roster when he is no longer suspended. The Packers kept four tight ends, who all have unique roles when they’re in the game. Tight End: Robert Tonyan, Marcedes Lewis, Josiah Deguara, Dominique Dafney (SUS: Jace Sternberger) Taylor likely got the nod due to his age and ability to contribute in the punting game as both a vice and punt gunner. If Begelton doesn’t make a 53-man roster somewhere, expect him to re-sign to the Packers’ practice squad, as he had a quality preseason for the team. Malik Taylor took a race for the final receiver spot to the finish line with Reggie Begelton, a former CFL all-star. Wide Receiver: Davante Adams, Marquez Valdes-Scantling, Allen Lazard, Randall Cobb, Amari Rodgers, Malik Taylorįive of the six receivers to make the team were essentially roster locks by the time the preseason started. If a running back should go down, Taylor could be activated and may stick the rest of the season on the roster. Taylor has practice squad potential that could lead to him contributing as a kick returner and as a non-ball-in-hand special teamer at some point this season. ![]() In a crowded backfield, Patrick Taylor and Dexter Williams were unable to make the final roster. Running Back: Aaron Jones, AJ Dillon, Kylin Hill ![]() Expect there to be an attempt to sign Kurt Benkert to the practice squad, with quarterbacks like Jake Dolegala and the twice-worked-out Donald Hammond being in the mix, if they can’t keep Benkert in Green Bay. The Packers officially go into the season with a two-man quarterback room, which probably should be the case when a former first-rounder flashed the way Jordan Love did this preseason. Without further ado, here are your 2021 Green Bay Packers with some analysis, broken down by position, on why certain players were kept, why certain players weren’t, and who could be in play for practice squad spots moving forward. ![]() ![]() Others try to pick the choice that best fits their entire playthrough and all the choices they’ve made regarding organics and synthetic life up until this point. ![]() Many players argue that the Destroy ending is the ‘better’ choice, considering how Shepard set out to destroy the Reapers from the start of the trilogy. You see them taking a breath, implying that they survived. Right before the ‘epilogue’, you see Shepard’s chest in the rubbles of the Crucible, only recognizable through their N7 armor. The rest of the game will play out like the regular Destroy ending, except for one of the very last scenes. To get this outcome, you must have at least 7800 EMS by the start of ‘Priority: Earth’, and you must pick the Destroy option. This is the only ending where Shepard survives. Mass Effect 3 Extended Cut | The Best Possible Destroy Ending - Shepard Lives The five different endings you can get in Mass Effect 3 are: Destroy Ending (Red) These options will vary depending on your EMS, and the choice you make will seal the fate of everyone in the galaxy. The Catalyst explains why the Reapers were created, before giving you the opportunity to make one last choice. Afterward, the Catalyst appears to you in the form of a child. There you find Captain Anderson, and confront the Illusive Man one last time. After fighting the Reapers in London, you are ‘beamed up’ to the Crucible. No matter how many war assets or EMS you have by ‘Priority: Earth’, the ending starts the same way for everyone. Complete ME2 with all your companions alive.Explore and scan as many planets as you can in all three games to find war assets.Get the Quarian and Geth to make peace in ME3.Whenever possible, keep people alive save everyone that can be saved, and get people to make peace.Complete as many missions and sidequests as possible. Here are some tips on how to optimize how much EMS you have: ![]() Please note that your choice regarding the Collector Base at the end of ME2 will impact which ending is available to you if you are very low on EMS by the end of the game. To unlock the best ending, you should aim to get at least 7800 EMS by the start of the final mission, ‘Priority: Earth’. Galaxy Readiness and multiplayer have been taken out of Mass Effect: Legendary Edition as a result, the amount of EMS needed by the end of the game has increased as well. The only way for players to increase their Galaxy Readiness was by playing multiplayer for a few hours. In the original game, EMS came hand-in-hand with Galaxy Readiness, which you had to get from 50% to 100% before the end of the game to get the best options. The higher your EMS, the more options you will have, with greater chances of avoiding tragic outcomes. Your amount of EMS grows as you get more war assets–usually people from different species working with you. In Mass Effect 3, what determines which ending you get is the amount of Effective Military Strength (EMS) you accumulate by the end of the game. This guide will explain how to get each possible ending. Just like with Mass Effect and Mass Effect 2, the choices you make throughout the game have an impact on how the game will end for you. In its remastered version, there are five different endings you can get in Mass Effect 3. ![]() ![]() ![]() Now, you can apply a preset to your photos by opening Layer > New Adjustment Layer > Presets. Click on it and import the needed presets. Once you’ve downloaded them to your computer, you need to open Affinity Photo, and find the “Add Preset” button in the upper left corner. You can also choose presets that enliven the color gamut or opt for filters aimed at B&W transformations.Īll presets are available on the FixThePhoto website, so you can be sure you get a licensed product. When using it to alter a picture, things seemed to go pretty well within the filter itself. ![]() ![]() Adding the plugin worked well, and it loaded easily when I asked it to. Filter Forge aims for quality and quantity, with over 13,400 photo effects and. there are a vast amount of texture filters in the filter forge library to choose from once I have my texture set the way I want it I make sure that seamless tiling is checked on and click apply. 1k Posted SeptemI downloaded Filter Forge 5 today (demo version) and installed it into both Affinity Photo 1.4.3 and the latest 1.5 beta. Photoshop, Capture One, Affinity Photo, and Corel Paint Shop Pro. Build your own filter with the great and powerful Filter Forge plugin to generate 3D render maps and create an Oz-inspired landscape, including the iconic. Then I select all (ctrl +A on Pc and I think cmd +A on mac) once I have my selection I go to the filers menu and select Filter forge from the plugins. Some presets in the package are suitable for editing portrait and family photos, while others reveal their full potential when applied to landscape and urban shots. Use Filter Forge and Adobe Photoshop to Create a Yellow Brick Road. They are claimed to cover a multitude of photo genres. These presets are easy to download and use in all Affinity Photo versions. Select the Brushed Stainless Steel and Beveled Mirrors by ronviers texture. Use the Search bar to search for some Brushed Metal textures. Filter Forge is a high-end Photoshop plugin that lets novice and expert users create a wide variety of realistic and. This will launch your web browser and direct it to Filter Forge's online gallery of textures and filters. It can be used as a standalone application or as a plugin for compatible 8bf hosts such as Adobe Photoshop, Affinity Photo, Corel PaintShop Pro. The plugin on the Mac platform is only compatible with Photoshop CC. They are designed to speed up a regular routine and provide newbie and professional photographers with efficient tools for creative experiments. Plugin di Filter Forge in Affinity Photo - YouTube Come installare il plugin di Filter Forge in Affinity Photo e cosa Filter Forgeil mio canale Telegram. Launch Filter Forge again and use the top link for Filter Library: Download more filters. Filter Forge is a computer graphics program for Windows and Mac that allows users to create procedural textures and modify images. NET and the latest version of Affinity Photo. The collection of free Affinity Photo presets can become a helpful addition to your standard color editing toolset. ![]() ![]() Dual gas tanks are fitted, with the rear tank reportedly disconnected and in need of cleaning and possible repair before use. then under a trestle bridge to hug Clipper Cove, then up behind the Chisholm Trail. The seller reports a newer exhaust has been added, and the front gas tank has been replaced. suspending the little camper between the burro's legs. Engine bay updates completed by the prior owner included a carb rebuild, and new fuel pump and filters, camshafts, cam chain, and lifters, radiator, hoses and belts. The 390ci V8 delivered 255hp when new, a 40hp boost over the original 360ci V8 which it replaced when this F-250 was near new. The white headliner show no marks or sagging, and the chrome surround is bright and nicely fitted. All lighting and gauges reported working correctly, including the original AM-only radio. The instrument binnacle is clean and shows only light hazing to plastic lenses. The dash includes a nice combination of painted metal, dark red vinyl, faux wood inserts and chromes accents. This example was delivered with power steering, but A/C is not fitted. The 4-speed manual gear box is floor shifted and reported to be in good working order. Dark red vinyl and upholstery remains in good shape, with no tears or cracking. The seller believes the interior to be unrestored, although some interior surfaces may have been included in the repaint as the painted body-color metal on the dash, doors and pillars looks newer. The rear bumper appears to be aftermarket with a matte steel base and diamond-plate step.Ī locking, passenger side under-bed toolbox is a cool and practical F-250 feature.Ī modern tonneau bed cover has clean black vinyl over a ribbed-metal base, dual prop sticks, and appears to fit well. The spray-in bedliner is a good color match and shows just a few scuffs. New outside mirrors were added when the truck was repainted. Painted steel wheels, chrome center caps, and Vitour Explorer A/T tires were all reportedly added as part of the refurb. Body panels appear straight, with good shut lines, and no evidence of damage, filler or repairs. Chrome is described as original and shiny, with a slight misalignment of the tailgate latch. The Red-over-White repaint appears to be have been done to a nice standard and remains shiny, with no obvious chips, cracking, color variation or fading. This Camper Special is on consignment from the current owner through the selling dealer who is representing the sale, and the car is offered with a clean Oregon title.į-Series’ 5th generation trucks were built on the same platform as the 4th gen but with updated styling, a larger cab and more engine choices. ![]() ![]() Recent service includes a full tune-up with oil/filter change in preparation for sale. This 3/4 ton truck benefits from a major refurbishment completed by a prior owner, including new paint, front gas tank, radiator and hoses, fuel pump, belts, exhaust system, camshafts and lifters, outside mirrors, wheels, tires and hub caps, and a carb rebuild. ![]() The original owner had the Ford dealer swap in a 6.4L 390ci V8 in placed of the original 360ci lump after just 3 months and for an unknown reason, and it is mated to a 4-speed manual. The Camper Special option package included heavy duty cooling, camper pre-wiring and a larger alternator. Now repainted in Red-over-White with original dark red vinyl interior, this 3-owner example has covered 67k miles. Gas bottle leg winder battery water can keys etc the seating area can make in to a double bed or the kitchen can pack down and the full caravan can make a huge bed the interior is the same as outside its absolutely immaculate the seating and curtains are unmarked never had pets inside or smokers it's absolutely beautiful and gets huge amounts of interest where ever we go I originally purchased it with the idea to tow behind my 1956 hillman minx but we have since decided that we have other commitments and just won't be a possibility anytime soon I will be sad to see it go as it is genuinely a 1 off in this condition I have all documentation and full custom fitted awning included in sale for further info please call.This 1969 Ford F-250 Camper Special remained under single-family ownership until 2014. Portafold ansfold 1960s/ 1970s folding caravan light weight designed to be towed by any car from a 850cc really easy to move around would look brilliant behind a mini I believe it's 350kg it as twin gas hobbs a sink & battery light pretty basic but plenty for simple caravanning holidays or steam rallies I purchased it from the original owner he had used it every year then stored it in he's garage when not in use and I have done the same the truly in immaculate condition with no damage damp or anything it tows perfectly good tyres + spare. ![]() ![]() ![]() After all, you never know - its power may be needed again. Austin Riley and Matt Olson hit back-to-back homers off Jacob deGrom in the second inning and Dansby Swanson later connected off the Mets ace as the Atlanta. We’ll have to see if Rosario brings that bat with him to Los Angeles. So, the last five times he's swung this bat, Rosario has hit for the cycle and delivered a game-winning hit in a #Postseason game. Published ApEddie Rosario’s HR gives Braves sweep of Reds Sportsnaut Credit: Dale Zanine-USA TODAY Sports Eddie Rosario hit a go-ahead solo home run in the eighth inning to. ![]() Eddie Rosario fell victim to baseballs economy when the Twins did not offer him a contract for 2021, making. Here it is: #Braves Eddie Rosario using his bat from the cycle (right) to get the game-winning hit against the Dodgers in Game 2 of the NLCS (left). Twins will move on without Eddie Rosario Berrios, Buxton sign. And as you can see, it actually was the same bat from that Sept. He got traded midseason of 2021 to Atlanta to rebuild their injured outfield. ![]() When Rosario returned with the bat, he only needed to see one pitch to end the game.ĪNOTHER DAY, ANOTHER #WALKOFF!!! /Jrn82L91Dk What a roller coaster the last couple of years have been for Rosario. "I went inside and I had the bat from the cycle saved to keep as a trophy. FOX Sports North (fsnorth) August 1, 2020. Scottie Pippen did a long, interesting interview with GQ in which he took aim at several current stars (and also Ben Simmons). It was Rosario’s fourth hit of the game, and it turned out that there was quite the story behind the bat he chose for the pivotal at-bat.Īs Dodgers closer Kenley Jansen was getting warmed up to enter the game, Rosario returned to the clubhouse to retrieve the bat from his Sept. An unidentified former Blackhawks player alleges in a lawsuit that he was sexually assaulted by an assistant coach in 2010. The Braves took a 2-0 lead in the National League Championship Series against the Dodgers on Sunday night when Rosario ripped a walk-off, line-drive single that Corey Seager couldn’t handle beyond second base. CLEVELAND, Ohio The Eddie Rosario who has been on display in the postseason for Atlanta is not the same Eddie Rosario who Cleveland fans watched for. Just ask Atlanta Braves outfielder Eddie Rosario. 'The only one I was not looking forward to facing was Berrios,' Rosario admitted. But sometimes, those superstitions pay off in a big way. Both hits came off close friend and fellow Puerto Rican Jose Berrios. For a game with so much failure, players will take luck wherever they can find it. It’s difficult to think of a more superstitious collection of athletes than professional baseball players. ![]() ![]() Use our advanced filters to find the vessel that best fits your style and expectations. The type of rentals available can vary from location to location, but you can expect anything from catamarans, sailboats, party boats and, of course, motor yacht charters. There are a few areas to choose for your sailing experience: Miami Beach, Downtown Miami, Key Biscayne, Haulover Sandbar, and Coconut Grove. Want to charter a luxury yacht but don't know where to start? We've got you covered. Make sure to check out our selection of weekly private charters available from Miami or Key West! Explore boat rental options in Miami Besides the day charters available for 2, 4 hours or an entire day, you can now book on Sailo an overnight trip or even a week-long sailing private cruise to the Florida keys. No matter what you choose to do in South Florida, the only way to truly capture the unique spirit of this place is by boat. Browse any pick your favorite from hundreds of Miami boat rentals and yacht charters! Week-long sailing vacations Whether you want to relax on a luxury yacht, reel in the big catch, or watch the breath-taking sunset on one of our many catamarans, motor boats and sailboat rentals in Miami, FL Sailo offers an unforgettable experience on the water. ![]() Now that you're here, it's time for you to find the perfect boat rental in Miami and explore our recommendations of things to do by boat in the city that never ceases to amaze its residents and visitors alike. Here, the city meets the seashore in an environment like no other: sailing in the Magic City, you'll delight in remarkable views of the city skyline amid bright blue water and an ocean breeze. With its remarkable art scene, delicious Cuban cuisine, and breathtakingly clear waters, there's no better place for an adventure on the water than Miami. Welcome to the yachting capital of the world! When it comes to sailing in south Florida, this is the place to be. ![]() ![]() ![]() Broad spectrum formulas: Broad spectrum formulas are the best for sun protection, so all items on our list have to shield from UVA and UVB rays.Bonus: I’ve even tried out some of these sunscreens and can pledge for the quality of the products. Customer ratings and reviews: In order to make our list, these products had to have glowing reviews from users.Here are some important factors that were taken into account: However, after years of testing and trying out products that mesh well with my skin type, I’d consider myself to be pretty well-versed in choosing oily skin-approved items, including sunscreens. Nearly every product that sits on my skin results in a slippery, slick mess within a few hours (you’re welcome for that visual). Beyond our skin care experts, I myself have ridiculously oily skin. With the help of our team of dermatologists here at Healthline, we’ve compiled a list of the best sunscreens for oily skin. Our dermatologists aren’t affiliated with any of the companies below. Keep in mind that, as with any skin care product, the process can entail a bit of trial and error until you find the sunscreen that works best with your skin. ![]() Healthline’s team of dermatology experts sifted through the sunscreen market to find the best products for oily skin. ![]() The key is to find the right products that won’t add more oil to your skin and lead to breakouts. You may assume this means that you shouldn’t wear sunscreen if you have oily skin, but every skin type needs sunscreen. The last thing you want is to add more oil to your skin with skin care products. Having oily skin means the sebaceous glands under your hair follicles are overactive and produce more sebum than usual. ![]() If your skin feels greasy and looks shiny a few hours after washing your face, you likely have oily skin.
![]() ![]() The central nervous system (CNS) is considered a site of refuge as cancers are protected from most systemic therapies by the blood–brain barrier (BBB). As overall survival in patients with primary non-CNS cancers has improved, the incidence of metastasis to the brain has also increased, possibly due to circulating cancer cells which persist despite patient remission. ![]() The highest incidence of brain metastasis is seen in lung cancer patients (40–50%) followed by breast (20–30%) and melanoma (5–10%). 20–40% of patients with systemic cancers develop secondary brain tumours. The majority of intracranial tumours are secondary metastases originating from primary non-central nervous system (non-CNS) cancers. The clinical significance of these studies includes the possible use of these epitopes as biomarkers for metastasis. Results from these studies suggest that overexpression of CD15 and CD15s could potentiate the transmigration of circulating NSCLC cells into the brain. Surprisingly, although the cells characterised as ‘non-metastatic’, they became ‘metastatic’ -like when cells were forced to over-express either FUT4 or FUT7. Knockdown of FUT4 and FUT7 in metastatic cancer cells prevented disruption of an in vitro BBB model. This overexpression led to the disruption of cerebral endothelial cell monolayers (p < 0.01). Overexpression of CD15 or CD15s epitopes led to increase in adhesion of cancer cells to cerebral endothelial cells compared with wild-type and cells with silenced CD15 or CD15s (p < 0.01). ![]() Two fucosyltransferases (FUT4 and 7) responsible for CD15 and CD15s synthesis were modulated in four human cancer cell lines (three lung cancer and one glioma). Three distinct, independent methods were used to measure brain endothelial integrity and include voltohmmeter (EVOM™), impedance spectroscopy (CellZscope®) and electric cell-substrate impedance sensing system (ECIS™). The aim of this work was to investigate the role of fucosylated carbohydrate epitopes CD15 and sialyated CD15s in cancer adhesion to brain-derived endothelial cells and determine their influence in blood–brain barrier (BBB) disruption Methods The mechanisms involved in how circulating cancer cells transmigrate into brain parenchyma are not fully understood. Metastatic non-small cell lung (NSCLC) cancer represents one of the most common types of brain metastasis. ![]() |
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